Heparin binding protein (HBP)

(latex immunoturbidimetric method)

ELISA Kit for Heparin Binding Protein (HBP)

----------- a new marker of acute bacterial infection, an early indicator of organ dysfunction in sepsis.

Biological characteristics of HBP:

Heparin-binding protein (Heparin-Binding Protein, or HBP)HBP is a protein secreted by mature neutrophils. Levels of HBP are elevated in cases of bacterial infection or acute respiratory distress syndrome (ARDS) or acute lung injury (ALI) and respiratory failure. HBP is not elevated in non-bacterial infections.

HBP is one of the earliest elevated markers of bacterial infection and can be detected in plasma within 1h after local or mild bacterial infection. This is an advantage over procalcitonin (PCT).

The level of HBP can also be applied to the identification of various types of post-surgical infections. [1

Structural characteristics of HBP

Heparin-binding protein is a neutrophil-derived granule protein. Its structure includes a single-chain protein of 222 amino acids, contains 8 cysteine residues, and has a glycosylation site at the 100th, 114 or 145 aspartic acid residue. The structure is similar to neutrophil elastin, and the homology is 45%.

 

 

Clinical significance of HBP:

In clinical, heparin binding protein can be used as a diagnostic index of local infection, auxiliary diagnosis of urinary tract infection, the prediction of septic shock and the prediction of microcirculatory failure in sepsis.

 

Comparison of HBP, PCT and CRP HBP: high sensitivity, strong specificity, high positive/negative detection rate, early infection can be detected, high concentration in acute bacterial infection, and low concentration in viral infection and non-specific inflammation. PCT: It can appear in the early stage of infection, which is increased when the body has a systemic response to infection, and the serum level is not significantly increased in local infection and chronic infection. CRP: A variety of infectious and non-infectious factors (surgery) can cause CRP to rise, which starts to rise 6-8h after infection, reaches the peak at 24-48h, and the specificity is not ideal.

Vertical top self-produced OEM inflammation series star product recommend

Product Name	Item No.
Heparin Binding Protein OEM Reagent	LD-OEM-HBP
Heparin-binding protein calibrator	LD-OEM-HBP-CAL
Heparin-binding protein controller	LD-OEM-HBP-QC
C- Reactive Protein OEM Reagent	LD-OEM-CRP
C- Reactive Protein Calibrator	LD-OEM-CRP-CAL
C- reactive protein control	LD-OEM-CRP-QC
Serum amyloid OEM reagent	LD-OEM-SAA
Serum amyloid calibrator	LD-OEM-SAA-CAL
serum amyloid protein control	LD-OEM-SAA-QC
Procalcitonin OEM Reagent	LD-OEM-PCT
Procalcitonin calibrator	LD-OEM-PCT-CAL
Procalcitonin Quality Control	LD-OEM-PCT-QC

Self-produced heparin-binding protein antigen recommend

Product Name	English abbreviation	Item No.
heparin binding protein antigen	HBP Ag	LD-Ag-HBP
HBP Antigen Diluent	HBP Dilution	LD-D-HBP-L

2-8 ℃ stability

　　

 

Thermal stability at 37 ℃

　　

 

The results showed that the deviation of heparin-binding protein antigen was within ± 10% at 4 ℃ and 37 ℃.

Self-produced Heparin Binding Protein Antibody recommend

Product Name	English abbreviation	Item No.
Mouse anti-human HBP monoclonal antibody 01	HBP mAb 01	LD-mAb-HBP-01
Mouse anti-human HBP monoclonal antibody 02	HBP mAb 02	LD-mAb-HBP-02

In the ELISA platform, the use of the company's antibodies can still achieve good response performance.

　　

 

After a large number of experimental studies have shown that the use of self-produced antibodies and recommend microspheres with labeling can achieve better labeling results.

standing top recommend microspheres

Product Name	English Name	Item No.
Microsphere 300	Latex300	LD-cLATEX-300

 

particle size	290-320 NM
carboxyl content	0.041-0.061mol/g
Solid content	10-10.5%

 

recommend process: one-step method

Reagent

Combined with market demand, Nanjing Liding Medical Technology Co., Ltd. after years of research, successfully developed heparin binding protein (latex immunoturbidimetry). The parameters and related performance are as follows:

Linear

　　

 

Precision

Reagent comparison:

　　

 

Stability at 4 ℃:

　　

 

Stability at 37°C:

　　

 

Open Bottle Stability:

　　

 

　　

 

　　HOOK：

　　

 

Technical Support

In addition to having a comprehensive antigen, antibody, and reagent development platform, LeadingMedcial are more capable of tailoring one-stop in vitro diagnostic product solutions, covering all aspects of registration, research and development, and production, and providing more comprehensive products and services.

This project can provide antigen antibody OEM reagent process transfer and other flexible service content!!!