Procalcitonin (PCT)

Project Introduction

PCT is a parameter for the diagnosis and monitoring of bacterial inflammatory disease infections. PCT can be used as an acute parameter to differentiate between bacterial and non-bacterial infectious inflammation, as a basis for monitoring patients at risk of infection (e. g., after surgery and organ transplantation during immunosuppression, after multiple trauma) and patients requiring intensive care; at the same time, to detect the systemic effects of bacterial infection or to detect septic complications, and to evaluate the clinical course and prognosis of severe inflammatory diseases.

Product Features

High sensitivity: functional sensitivity of PCT reagent ≤ 0.3ng/mL.

Rigorous traceability: The traceability system is established through the primary calibrator PTN47 of procalcitonin established by BRAHMS.

Mass production upgrade: The unique "one-pot cooking" process makes it easy to supply thousands of upgraded reagents in a single batch.

Excellent correlation: 50 samples of Roche chemiluminescence were tested, and the correlation coefficient r≥ 0.95.

5) good compatibility: compatible with a variety of serum, plasma, whole blood, compatible with all kinds of biochemical analyzer, specific protein analyzer, blood cell analyzer and other instruments.

Vertical Top Self-produced OEM Inflammation Series Star Reagent recommend

Item No.	Reagent ratio	Reference Range	linear range
C- reactive protein (CRP)
LD-hsPCT-01	R1:R2=3:1	0-0.5 ng/ml		0-20 ng/ml
LD-PCT-01	R1:R2=3:1	0-0.5 ng/ml		0-60 ng/ml
LD-CRP-S	Customizable
Serum amyloid A(SAA)
LD-SAA-OEM	R1:R2=4:1	0-10 mg/L		2-300 mg/L
LD-SAA-S	Can be customized according to the machine
C- reactive protein (CRP)
LD-CRP-01	R1:R2=4:1	0-10 mg/L		2-200 mg/L
LD-CRP-02	R1:R2=1:1	0-5 mg/L		0.5-320 mg/L
LD-CRP-S	Customizable
Heparin-binding protein (HBP)☆
LD-HBP-OEM	R1:R2=4:1	0-10ng/ml	2-200 ng/ml

PCT antigen

The company through the recombinant expression of natural PCT protein, developed a stable recombinant PCT protein, with the company's supporting antigen preservation liquid, can ensure that the protein in the liquid case, at 37 degrees C heat accelerated for 7 days, protein activity loss does not exceed 10%.

Following the company's procalcitonin (PCT) detection kit (latex enhanced immunoturbidimetric method), the stability of the antigen was tested, the results show that the accelerated investigation at 37 ℃ for 7 days, the deviation is within 10%; 2-8 ℃ conditions for 2 months, the deviation is within 10%.

Thermal stability at 37 ℃

　　

 

2-8 ℃ stability

　　

 

　Vertical top self-produced PCT antigen recommend:

Product Name	English abbreviation	Item No.
PCT antigen☆	PCT Ag	LD-Ag-PCT
PCT antigen dilution☆	PCT Dilution	LD-D-PCT-L

PCT antibody

Product Name	English abbreviation	Item No.
Mouse anti-human PCT monoclonal antibody 01☆	PCT mAb 01	LD-mAb-PCT-01
Mouse anti-human PCT monoclonal antibody 02☆	PCT mAb 02	LD-mAb-PCT-02
Mouse anti-human PCT monoclonal antibody 03☆	PCT mAb 03	LD-mAb-PCT-03
Mouse anti-human PCT polyclonal Antibody 04☆	PCT gpAb 04	LD-gpAb-PCT-04

The use of the company's antibodies can be directly adapted to the use of the company's latex microspheres, the effect is better!

Product Name	English abbreviation	Item No.
Microsphere 400	Latex 400	LD-cLATEX-400

 

particle size	380 -420 NM
carboxyl content	0.08-0.10mmol/g
Solid content	10.0-10.5 %

 

recommend process

　　

 

Reagent performance

Top product features:

Name	LeadingBio
linear range	0.5-50 ng/ml
Adapt parameters	3:1 etc (R1/R2)
Sample Type	serum
Precision	Intra-batch CV ≤ 5.0%; inter-batch relative deviation R≤ 10.0%.
Other	At the same time can provide supporting calibration, quality control and antigen antibody

Linear:

　　

 

Precision:

	inter-batch precision		In-batch precision
(Ng/ml)	Low	High	Low	High
N	10 (batch)	10 (batch)	20 (times)	20 (times)
AVE	0.45	30.2	0.49	31.1
CV	4.7%	1.4%	3.0%	0.9%

The inter-batch and intra-batch precision of reagents performed well.

Reagent comparison:

　　

 

Good clinical consistency of reagents and imported reagents

Stability at 37°C

　　

 

2-8 ℃ stability:

　　

 

2-8 degrees Celsius open bottle stability

　　

 

The reagent has good stability, and the deviation is within 10% under the condition of 37 ℃ for 7 days. The deviation is within 10% when the bottle is opened at 2-8 ℃ for 1 month.

　　HOOK：

　　

 

Reagent hook critical concentration ≥ 70ng/mL