Adiponectin (ADP)

Project Introduction

adiponectin biological characteristics

Adiponectin (Adiponectin, ADP,ADPN,ADN) is a hormone secreted by adipocytes and is thought to exist in the blood circulation in different forms, of which multimers are the main active form. It has the physiological functions of regulating glucose metabolism, anti-inflammatory response, anti-atherosclerosis and so on. It is a comprehensive index to reflect the body's physiological and metabolic health, and has important value for early risk screening, condition monitoring and prognosis evaluation of type II diabetes.

structural characteristics of adiponectin

Adiponectin consists of 244 amino acids and has a molecular weight of approximately 30 kDa(28-30 kDa). The adiponectin molecule has four domains: the N-terminal secretory signal sequence (aa 1-18), the specific sequence (aa19-41), the collagen repeat sequence (aa 42-107), and the globular sequence (aa108-244). The globular region is the key site of the biological activity of adiponectin.

　　

 

Structure of human and murine adiponectin

　　

 

globular region of adiponectin

Clinical significance of adiponectin

Adiponectin is an important regulator of lipid and carbohydrate metabolism, with anti-diabetic, anti-inflammatory and anti-atherosclerosis properties. Studies have shown that in the case of clinical symptoms such as obesity, type II diabetes (T2DM), cardiovascular disease, and dyslipidemia, a significant decrease in adiponectin levels can screen out high-risk people in the pre-diabetes period 4-7 years in advance. This stage is the reversible stage of the natural course of diabetes, which can effectively prevent and control the occurrence of diabetes and realize the primary prevention of type II diabetes.

　　

 

Academic Recognition

Four well-known prospective cohort studies in the world, including the Hoorn study, the Atherosclerosis Risk in Communities (ARIC), the Nurses 'Health study, the largest and most authoritative study on risk factors for chronic diseases in women in the world, and the European Nutrition and Cancer Risk Prospective Study (EPIC), have reached the same conclusion: the lower adiponectin, the higher the risk of diabetes. Therefore, adiponectin can be used as a new index to predict the early risk of diabetes.

　　Vertical top self-produced OEM diabetes series star reagent recommend:

Product Name	Item No.
Glycosylated Hemoglobin OEM Reagent ☆	LD-OEM-HbA1c
Glycosylated hemoglobin calibrator ☆	LD-OEM-HbA1c-CAL
Glycosylated hemoglobin control ☆	LD-OEM-HbA1c-QC
Adiponectin OEM Reagents ☆	LD-OEM-ADP
Adiponectin calibrator ☆	LD-OEM-ADP-CAL
Adiponectin quality control products ☆	LD-OEM-ADP-QC

 

International academic consensus: adiponectin can predict the risk of diabetes
Li S, Shin HJ, Ding EL, from Dam RM.JAMA. 2009 8;302(2):179-88. (IF:)	Daimon M, Oizumi pokemon pinata, Saitoh pokemon pinata, et al;Diabetes Care.2003;26(7):2015-2020.
Lindsay RS, Funahashi pokemon pinata, Hanson RL, et al.Lancet.2002;360(9326):57-58.	Duncan BB, Schmidt MI, Pankow JS, et al.Diabetes.2004;53(9):2473-2478.
Spranger J, Kroke A, Möhlig M, et al.Lancet.2003;361(9353):226-228.	Snijder MB, Heine RJ, Seidell JC, et al.Diabetes Care.2006;29(11):2498-2503.
Heidemann C, Sun Q, van Dam RM, et al.Ann Intern Med.2008;149(5):307-316.	Jiang Y, Owei I, et al.BMJ Open Diabetes Res Care.2016 17;4(1):e000194.
Koenig W, Löwel H, et al. J Am Coll Cardiol.2006;48(7):1369-1377.	Lai H, Lin N, Xing Z, Weng H, Zhang H.J Diabetes Investigate.2015 ;6(4):416-29.
SneAlibabatha C, Ramachandran A, et al.Diabetes Care.2003;26(12):3226-3229.

adiponectin antigen

As a prediction and monitoring index of type II diabetes, some manufacturers have begun to develop relevant detection kits in recent years. However, due to the particularity of adiponectin protein structure, it is easy to form different degrees of multimers, which has caused some problems for many manufacturers to develop reagents. Among them, the stability of liquid calibration is a key problem. After years of research, Nanjing Liding Medical Technology Co., Ltd. has greatly improved the liquid stability of adiponectin antigen by mutating the protein sequence and optimizing the storage buffer.

The stability of the antigen was tested by using the adiponectin detection kit (latex enhanced immunoturbidimetric method) developed by Nanjing Liding Medical Technology Co., Ltd. The results showed that the adiponectin antigen was frozen and thawed 4 times at -40 ℃, and the deviation was within 5%. Accelerate the investigation for 7 days at 37 ℃, and the deviation is within 10% (compare with imported antigen at the same time, the one-day deviation of acceleration at 37 ℃ is more than 10%, and it continues to decrease with the increase of time); The deviation is within 10% when stored at 2-8 ℃ for 2 months.

freeze-thaw stability

　　

 

Thermal stability at 37 ℃

　　

 

2-8 ℃ stability

　　

 

standing top from the production of adiponectin antigen recommend:

Product Name	English abbreviation	Item No.	Specifications
adiponectin antigen☆	ADP Ag	LD-Ag-ADP	0.1mg / 1mg/ 10mg
Adiponectin Antigen Diluent☆	ADP Dilution	LD-D-ADP-L	1ml/10ml/100ml

　antibody selection

Standing top self-produced diabetes series antibody recommend:

Product Name	English abbreviation	Item No.
Adiponectin Monoclonal Antibody 1☆	ADP mAb 01	LD-mAb-ADP-01
Adiponectin Monoclonal Antibody 2☆	ADP mAb 02	LD-mAb-ADP-02

　　

 

The use of the company's antibodies can be directly adapted to the use of the company's latex microspheres, the effect is better!

Product Name	English abbreviation	Item No.
Microsphere 180	Latex 180	LD-pLATEX-180

 

particle size	180-200 NM
carboxyl content	0.08-0.10
Solid content	10-10.5%

 

recommend process

　　

 

Reagent performance

After years of research, Nanjing Liding Medical Technology Co., Ltd. successfully launched adiponectin detection kit (latex enhanced immune turbidimetry). The parameters and related performance are as follows:

Name	ADP
Methodology	latex enhanced immunoturbidimetric method
Reagent ratio	R1/S/R2：160/3/40
Analysis Type	End point method
sub/main wavelength	0/700nm
linear range	2-40 mg/L
Sample Type	serum

Linear:

　　

 

Precision:

	inter-batch precision		In-batch precision
（mg/L）	Low	High	Low	High
Modification4:N	5(batch)	5(batch)	20Times	20Times
AVE（mg/L）	4.6	21.1	4.4	20.1
CV	2.1%	3.2%	1.3%	0.7%

Reagent comparison:

　　

 

Stability at 37°C:

　　

 

Stability at 4 ℃:

　　

 

Open Bottle Stability:

　　

 

The reagent has good stability, accelerated inspection at 37 degrees C for 7 days, the deviation is within 10%;

Store for 18 months, the deviation is within ± 10%; store for 1 month under 2-8 ℃ open condition, the deviation is within ± 10%.

　　HOOK：

　　

 

Technical Services

In addition to a comprehensive antigen, antibody and reagent development platform, Leading Medcial is more capable of tailoring one-stop solutions for in vitro diagnostic products, covering registration, research and development, production and other links, providing more comprehensive products and services.

This project can provide antigen antibody OEM reagent process transfer and other flexible service content!!!