Pepsinogen II(PGII)

Project Introduction

biological characteristics of pepsinogen

Pepsinogen (Pepsinogen, referred to as PG) is a precursor of pepsin, according to the biochemical properties and immunogenicity is divided into 2 subgroups, for pepsinogen I ( PG I) and pepsinogen II (PGII). About 1% of pepsinogen enters the blood and exists stably. The content of PG I and PG II in serum reflects the number of gastric mucosal glands and cells, and also indirectly reflects the secretory function of different parts of gastric mucosa. PG index in various gastric diseases have different degrees of change, to provide reliable diagnostic value for clinical.

　Clinical significance of pepsinogen

There are two subtypes of pepsin, PGI and PGII, and their levels reflect the morphology and function of gastric mucosa in different parts. PGI is a pointer to detect the function of gastric acid secreting gland cells. Increased gastric acid secretion, increased PGI, decreased secretion or gastric mucosal gland atrophy, decreased PGI; PGII is more correlated with gastric fundus mucosal lesions (relative to gastric antral mucosa), and its increase is related to gastric fundus gland duct atrophy, gastric epithelial metaplasia or pseudopyloric metaplasia, and heterotypic appreciation. Progressive decrease in PGI/II ratio is related to progression of gastric mucosal atrophy.

Pepsinogen (PG) on the development of gastric diseases, generally can be expressed as superficial gastritis-gastric mucosal erosion ulcer-atrophic gastritis-gastric cancer, and other diseases have a good diagnostic and screening role. PG I/II is used to detect the content of pepsinogen I/II in serum or plasma. Through the different serum measurement values, there are different degrees of changes in various gastric diseases, which provides reliable diagnostic value for clinical practice.

Vertical top self-produced gastric function series star reagent recommend:

Product Name	English abbreviation	Item No.
Pepsinogen I OEM Reagent ☆	PGI	LD - PGI
Pepsinogen II OEM Reagent ☆	PGII	LD - PGII
Pepsinogen I calibrator ☆	PGI Cal	LD-CAL-PGI
Pepsinogen II calibrator ☆	PGII Cal	LD-CAL-PGII
Pepsinogen I control substance ☆	PGI With	LD-QC-PGI
Pepsinogen II controls ☆	PGII With	LD-QC-PGII

PG antigen

As the prediction and monitoring index of atrophic gastritis and gastric cancer, PGI and PGI/PGII ratio are important detection results. The project uses composite calibrators and quality control products. That is, two different antigens, of which pairing recognition with the antibody in the reagent is a big problem. After years of research, Nanjing Liding Medical Technology Co., Ltd. has greatly improved the adaptation of pepsinogen antigen by mutating the protein sequence and optimizing the storage buffer.

In the following, the stability of the antigen was tested by using the pepsinogen (PG) detection kit (latex enhanced immunoturbidimetry) developed by Nanjing Liding Medical Technology Co., Ltd. The results showed that PGI and PGII antigens were accelerated for 7 days at 37 ℃, and the deviation was within 10%. Store at 2-8 ℃ for 2 months with a deviation within 10%.

PGI Antigens:

37 ℃ thermal stability:

　　

 

2-8 ℃ stability:

　　

 

PGII Antigens:

37 ℃ thermal stability:

　　

 

2-8 ℃ stability:

　　

 

standing top produced PG antigen recommend:

Product Name	English abbreviation	Item No.
Pepsinogen I ☆	PGI Ag	LD-Ag-PGI
Pepsinogen II ☆	PGII Ag	LD-Ag-PGII

antibody selection

Vertical top self-produced gastric function series antibody recommend:

Product Name	English abbreviation	Item No.
Mouse anti-human PGI monoclonal antibody 01 ☆	PGI mAb 01	LD-mAb-PGI-01
Mouse anti-human PGI monoclonal antibody 02 ☆	PGI mAb 02	LD-mAb-PGI-02
Mouse anti-human PGII monoclonal antibody 01 ☆	PGII mAb 01.	LD-mAb-PGII-01
Mouse anti-human PGII monoclonal antibody 02 ☆	PGII mAb 02	LD-mAb-PGII-02

 

The use of the company's antibodies can be directly adapted to the use of the company's latex microspheres, the effect is better!

PGI reagent microspheres recommend:

Product Name	English abbreviation	Item No.
Microsphere 300	Latex 300	LD-cLATEX-300

 

particle size	290-320 NM
carboxyl content	0.041-0.061 mol/g
Solid content	10-10.5%

 

PGII reagent microspheres recommend:

Product Name	English abbreviation	Item No.
Microsphere 400	Latex 400	LD-cLATEX-400

 

particle size	380 -400 NM
carboxyl content	0.08-0.10 mol/g
Solid content	10-10.5%

 

recommend process

　　

 

Reagent performance

After years of research, Nanjing Liding Medical Technology Co., Ltd. has successfully launched pepsinogen I(PGI) and pepsinogen II(PGII) detection kits (latex enhanced immunoturbidimetry). The parameters and related performance are as follows:

Name	PGI
Methodology	latex enhanced immunoturbidimetric method
Reagent ratio	R1/S/R2：162/6/30
Analysis Type	End point method
sub/main wavelength	0/700nm
linear range	2.5-300 ng/ml
Sample Type	serum

Linear:

　　

 

Precision:

	inter-batch precision		In-batch precision
(Ng/ml)	Low	High	Low	High
N	5 (batch)	5 (batch)	20 times	20 times
AVE(ng/mL)	25.33	75.22	25.02	74.36
CV	3.1%	2.2%	2.5%	1.4%

Reagent comparison:

　　

 

Good clinical consistency of reagents and imported reagents

Stability at 37°C:

　　

 

2-8 ℃ stability:

　　

 

2-8 ℃ open bottle stability:

　　

 

The reagent has good stability, accelerated inspection at 37 degrees C for 15 days, the deviation is within 10%;

Store for 18 months, the deviation is within ± 10%; store for 1 month under 2-8 ℃ open condition, the deviation is within ± 10%.

　　HOOK：

　　

 

Name	PGII
Methodology	latex enhanced immunoturbidimetric method
Reagent ratio	R1/S/R2：162/6/30
Analysis Type	End point method
sub/main wavelength	0/700nm
linear range	2-100 ng/ml
Sample Type	serum

Linear:

　　

 

Precision:

	inter-batch precision		In-batch precision
(Ng/ml)	Low	High	Low	High
N	5 (batch)	5 (batch)	20 times	20 times
AVE(ng/mL)	10.33	33.22	10.92	33.20
CV	6.1%	5.2%	1.51%	0.82%

Reagent comparison:

　　

 

Good clinical consistency of reagents and imported reagents

Stability at 37°C:

　　

 

2-8 ℃ stability:

　　

 

2-8 ℃ open bottle stability:

　　

 

The reagent has good stability, accelerated inspection at 37 degrees C for 21 days, the deviation is within 10%;

Store for 18 months, the deviation is within ± 10%; store for 1 month under 2-8 ℃ open condition, the deviation is within ± 10%.

　　HOOK：

　　

 

Technical Services

In addition to having a comprehensive antigen, antibody, and reagent development platform, LeadingMedcial are more capable of tailoring one-stop in vitro diagnostic product solutions, covering all aspects of registration, research and development, and production, and providing more comprehensive products and services.

This project can provide antigen antibody OEM reagent process transfer and other flexible service content!!!