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Pepsinogen II (PGII)

Number: LD-PGII-01 (mainly recommended version)
Keyword:
Pepsinogen
Product description

Project Introduction

1

Pepsinogen Biological Properties

Pepsinogen (PG) is a precursor of pepsin, which is divided into two subgroups according to biochemical properties and immunogenicity, namely pepsinogen I (PGⅠ) and pepsinogen II (PGII). About 1% of pepsinogen enters the blood and exists stably. The content of PG I and PG II in serum reflects the number of glands and cells in the gastric mucosa, and also indirectly reflects the secretory function of different parts of the gastric mucosa. PG indicators have different degrees of changes in various gastric diseases, providing reliable diagnostic value for clinical practice.

Clinical significance of pepsinogen

There are two subtypes of pepsin, PGI and PGII, and their levels reflect the morphology and function of gastric mucosa in different parts. PGI is an indicator to detect the function of oxyntic gland cells. Gastric acid secretion increases and PGI increases, while secretion decreases or gastric mucosal gland atrophy decreases PGI; PGII has a greater correlation with gastric fundus mucosal lesions (relative to gastric antrum mucosa), and its increase Higher levels were associated with atrophy of fundic gland ducts, gastric epithelial metaplasia or pseudopyloric gland metaplasia, and atypia; the progressive decrease of PGI/II ratio was associated with the progression of gastric mucosal atrophy.

The development process of pepsinogen (PG) on gastric diseases can generally be expressed as superficial gastritis - gastric mucosal erosion ulcer - atrophic gastritis - gastric cancer, and other diseases have a good diagnosis and screening effect. PGⅠ/Ⅱ is used to detect the content of pepsinogen Ⅰ/Ⅱ in serum or plasma. Through the difference of its serum measurement value, there are different degrees of changes in various gastric diseases, providing reliable diagnostic value for clinical practice.

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Liding self-produced gastric function series star reagents are recommended:

product name

English abbreviation

article number

Pepsinogen I OEM reagent☆

PGI

LD - PGI

Pepsinogen II OEM reagent☆

PGII

LD-PGII

Pepsinogen I calibrator☆

PGI Cal

LD-CAL-PGI

Pepsinogen II Calibrator☆

PGII Cal

LD-CAL-PGII

Pepsinogen I quality control product☆

PGI Con

LD-QC-PGI

Pepsinogen II quality control product☆

PGII Con

LD-QC-PGII

PG antigen

As predictive and monitoring indicators of atrophic gastritis and gastric cancer, PGI and the ratio of PGI/PGII are important detection results. This project uses compound calibrators and quality controls. That is, two different antigens, among them, paired recognition with the antibody in the reagent is a big problem. After years of research, Nanjing Liding Medical Technology Co., Ltd. has greatly improved the adaptation problem of pepsinogen antigen through mutational modification of protein sequence and optimization of storage buffer.

The following uses the pepsinogen (PG) detection kit (latex enhanced immune turbidimetry) developed by Nanjing Liding Medical Technology Co., Ltd. to test the stability of the antigen. The results show that the PGI and PGII antigens are at 37 ℃. Under accelerated investigation for 7 days, the deviation is within ±10%; 2-8 ℃ storage conditions for 2 months, the deviation is within ±10%.

PGI antigens:

Thermal stability at 37°C:

 

1

 

2-8℃ Stability:

 

1

 

PGII antigen:

Thermal stability at 37°C:

 

1

 

2-8℃ Stability:

 

1

 

Liding self-produced PG antigen recommendation:

product name

English abbreviation

article number

Pepsinogen I☆

PGI Ag

LD-Ag-PGI

Pepsinogen II☆

PGII Ag

LD-Ag-PGII

Antibody selection

Liding self-produced gastric function series antibodies are recommended:

product name

English abbreviation

article number

Mouse anti-human PGI monoclonal antibody 01☆

PGI mAb 01

LD-mAb-PGI-01

Mouse anti-human PGI monoclonal antibody 02☆

PGI mAb 02

LD-mAb-PGI-02

Mouse anti-human PGII monoclonal antibody 01☆

PGII mAb 01

LD-mAb-PGII-01

Mouse anti-human PGII monoclonal antibody 02☆

PGII mAb 02

LD-mAb-PGII-02

 

Using our company's antibodies can be directly adapted to our company's latex microspheres, and the effect is better!

PGI reagent microsphere recommendation:

product name

English abbreviation

article number

Microspheres 300

Latex 300

LD-cLATEX-300

 

particle size

290-320 nm

Carboxyl content

0.041-0.061 mol/g

solid content

10-10.5%

 

PGII reagent microsphere recommendation:

product name

English abbreviation

article number

Microspheres 400

Latex 400

LD-cLATEX-400

 

particle size

380-400 nm

Carboxyl content

0.08-0.10 mol/g

solid content

10-10.5%

 

Recommended process

 

1

 

Reagent performance

After years of research, Nanjing Liding Medical Technology Co., Ltd. has successfully launched pepsinogen I (PGI) and pepsinogen II (PGII) detection kits (latex-enhanced immune turbidimetry). The parameters and related properties are as follows:

name

PGI

Methodology

latex-enhanced immune turbidimetry

Reagent ratio

R1/S/R2: 162/6/30

Analysis type

endpoint method

Secondary/Main wavelength

0/700nm

Linear range

2.5-300ng/mL

sample type

serum

Linear:

 

1

 

Precision:

 

Inter-assay precision

within-assay precision

(ng/mL)

Low

High

Low

High

N

5 (batch)

5 (batch)

20 times

20 times

AVE (ng/mL)

25.33

75.22

25.02

74.36

CV

3.1%

2.2%

2.5%

1.4%

Reagent comparison:

 

1

 

Good clinical consistency between reagents and imported reagents

37℃ stability:

 

1

 

2-8℃ Stability:

 

1

 

2-8℃ open bottle stability:

 

1

 

The reagent has good stability, accelerated investigation at 37°C for 15 days, the deviation is within ±10%; 2-8°C condition guarantees

Stored for 18 months, the deviation is within ±10%; stored at 2-8 °C for 1 month, the deviation is within ±10%.

HOOK:

 

1

 

name

PGII

Methodology

latex-enhanced immune turbidimetry

Reagent ratio

R1/S/R2: 162/6/30

Analysis type

endpoint method

Secondary/Main wavelength

0/700nm

Linear range

2-100ng/mL

sample type

serum

Linear:

 

1

 

Precision:

 

Inter-assay precision

within-assay precision

(ng/mL)

Low

High

Low

High

N

5 (batch)

5 (batch)

20 times

20 times

AVE (ng/mL)

10.33

33.22

10.92

33.20

CV

6.1%

5.2%

1.51%

0.82%

Reagent comparison:

 

1

 

Good clinical consistency between reagents and imported reagents

37℃ stability:

 

1

 

2-8℃ Stability:

 

1

 

2-8℃ open bottle stability:

 

1

 

The reagent has good stability, accelerated investigation at 37°C for 21 days, the deviation is within ±10%; 2-8°C condition guarantees

Stored for 18 months, the deviation is within ±10%; stored at 2-8 °C for 1 month, the deviation is within ±10%.

HOOK:

 

1

 

Technical Services

In addition to having a comprehensive antigen, antibody, and reagent development platform, LeadingMedcial is more capable of customizing one-stop in vitro diagnostic product solutions, covering registration, R&D, production and other links, providing more comprehensive products and services.

This project can provide flexible service content such as antigen + antibody + OEM reagent + process transfer!!!

 

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