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Heparin-binding protein (HBP)

Item number: LD-HBP-03 (mainly recommended version)
Keyword:
Heparin-binding protein (HBP)
Product description

(Latex immunoturbidimetric method)

Heparin Binding Protein (HBP) Detection Kit

------------- Novel acute bacterial infection marker, early indicator of sepsis organ dysfunction.

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Biological properties of HBP:

Heparin-Binding Protein (HBP) HBP is a protein secreted by mature neutrophils, when bacterial infection or acute respiratory distress syndrome (ARDS) or acute lung injury (ALI) and respiratory failure occur , its level will increase. Nonbacterial infections do not raise HBP.

HBP is one of the earliest elevated markers of bacterial infection and can be detected in plasma within 1 h of localized or mild bacterial infection. This is an advantage over procalcitonin (PCT).

The level of HBP can also be used to identify various types of postoperative infections. [1

Structural features of HBP

Heparin-binding protein is a neutrophil-derived granule protein whose structure consists of a 222-amino acid single-chain protein containing 8 cysteine ​​residues with aspartic acid residues at positions 100, 114, or 145. Glycosylation site, structurally similar to neutrophil elastin, with 45% homology.

 

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Clinical significance of HBP:

In clinical, heparin-binding protein can be used as a diagnostic index of local infection, auxiliary diagnosis of urinary tract infection, prediction of septic shock and prediction of microcirculatory failure in sepsis.

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Comparison of HBP, PCT, CRP HBP: high sensitivity, strong specificity, high positive/negative detection rate, can be detected in the early stage of infection, high concentration in acute bacterial infection, and high concentration in viral infection and non-specific inflammation Low. PCT: It can appear in the early stage of infection, and it increases when the body's infection produces a systemic response. The serum level does not increase significantly during local infection and chronic infection. CRP: A variety of infectious and non-infectious factors (surgery) can cause CRP to rise. It begins to rise 6-8 hours after infection, and reaches a peak at 24-48 hours. The specificity is not ideal.

Liding self-produced OEM inflammation series star product recommendation

product name

article number

Heparin Binding Protein OEM Reagent

LD-OEM-HBP

Heparin-binding protein calibrator

LD-OEM-HBP-CAL

Heparin-binding protein control

LD-OEM-HBP-QC

C-reactive protein OEM reagent

LD-OEM-CRP

C-reactive protein calibrator

LD-OEM-CRP-CAL

C-reactive protein controls

LD-OEM-CRP-QC

Serum Amyloid OEM Reagent

LD-OEM-SAA

Serum Amyloid Calibrator

LD-OEM-SAA-CAL

Serum Amyloid Control

LD-OEM-SAA-QC

Procalcitonin OEM Reagent

LD-OEM-PCT

Procalcitonin Calibrator

LD-OEM-PCT-CAL

Procalcitonin Quality Control

LD-OEM-PCT-QC

Liding self-produced heparin-binding protein antigen recommendation

product name

English abbreviation

article number

heparin binding protein antigen

HBP Ag

LD-Ag-HBP

HBP Antigen Diluent

HBP Dilution

LD-D-HBP-L

2-8℃ Stability

  

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37℃ thermal stability

  

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The results showed that the deviation of the heparin-binding protein antigen at 4℃ and 37℃ was within ±10%.

Liding self-produced heparin-binding protein antibody recommendation

product name

English abbreviation

article number

Mouse anti-human HBP monoclonal antibody 01

HBP mAb 01

LD-mAb-HBP-01

Mouse anti-human HBP monoclonal antibody 02

HBP mAb 02

LD-mAb-HBP-02

On the ELISA platform, good reaction performance can still be achieved using our antibodies.

  

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After a large number of experimental studies, it has been shown that better labeling results can be achieved by using the self-produced antibody and the recommended microspheres for labeling.

Standing top recommended microspheres

product name

English name

article number

Microspheres 300

Latex300

LD-cLATEX-300

 

particle size

290-320 nm

Carboxyl content

0.041-0.061mol/g

solid content

10-10.5%

 

Recommended process: one-step method

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reagent

Combined with market demand, Nanjing Liding Medical Technology Co., Ltd. has successfully developed heparin-binding protein (latex immunoturbidimetric method) after years of research. The parameters and related properties are as follows:

name

Methodology

Reagent ratio

Analysis type

Secondary/Main wavelength

Linear range

sample type

HBP

latex-enhanced immune turbidimetry

R1/S/R2: 160/12/40

endpoint method

0/546nm

2-200ng/ml

Sodium citrate plasma

linear

  

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Precision

 

Inter-assay precision

within-assay precision

(ng/mL)

Low

High

Low

High

N

5

5

20

20

AVE

19.5

91.2

19.9

94.1

CV

2.8%

3.8%

2.0%

1.2%

Reagent comparison:

  

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4℃ Stability:

  

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37℃ stability:

  

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Open bottle stability:

  

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  HOOK:

  

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Technical Support

In addition to having a comprehensive antigen, antibody, and reagent development platform, LeadingMedcial is more capable of customizing one-stop in vitro diagnostic product solutions, covering registration, R&D, production and other links, providing more comprehensive products and services.

This project can provide flexible service content such as antigen + antibody + OEM reagent + process transfer!!!

  

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We could not find any corresponding parameters, please add them to the properties table
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