Project Introduction
PCT is a parameter for the diagnosis and monitoring of bacterial inflammatory disease infections. PCT can be used as an acute parameter to differentiate between bacterial and non-bacterial infections and inflammation, as a basis for monitoring patients at risk for infection (eg, post-surgery and post-organ transplant periods of immunosuppression, post-multiple trauma) and Patients requiring intensive care; at the same time, to detect the systemic impact of bacterial infection or to detect septic complications, to evaluate the clinical course and prognosis of severe inflammatory disease.
Features
High sensitivity: The functional sensitivity of PCT reagent is less than or equal to 0.3ng/mL.
Rigorous traceability: The traceability system is established through the primary calibrator of procalcitonin PTN47 established by BRAHMS.
Mass production upgrade: The unique "one-pot cooking" process makes it easy to supply thousands of liters of other reagents in a single batch.
Excellent correlation: 50 samples of Roche chemiluminescence were tested, and the correlation coefficient r≥0.95.
5) Good compatibility: Compatible with a variety of serum, plasma, whole blood, compatible with various biochemical analyzers, specific protein analyzers, blood cell analyzers and other instruments.
Liding self-produced OEM inflammation series star reagent recommendation
article number |
Reagent ratio |
Reference range |
Linear range |
|
C-reactive protein (CRP) |
||||
LD-hsPCT-01 |
R1:R2=3:1 |
0-0.5ng/mL |
0-20ng/mL |
|
LD-PCT-01 |
R1:R2=3:1 |
0-0.5ng/mL |
0-60ng/mL |
|
LD-CRP-S |
customizable |
|||
Serum Amyloid A (SAA) |
||||
LD-SAA-OEM |
R1:R2=4:1 |
0-10 mg/L |
2-300 mg/L |
|
LD-SAA-S |
Can be customized according to the machine |
|||
C-reactive protein (CRP) |
||||
LD-CRP-01 |
R1:R2=4:1 |
0-10 mg/L |
2-200 mg/L |
|
LD-CRP-02 |
R1:R2=1:1 |
0-5 mg/L |
0.5-320 mg/L |
|
LD-CRP-S |
customizable |
|||
Heparin-binding protein (HBP)☆ |
||||
LD-HBP-OEM |
R1:R2=4:1 |
0-10ng/mL |
2-200ng/mL |
PCT antigen
Through the recombinant expression of natural PCT protein, the company has developed a recombinant PCT protein with excellent stability, and with the company's supporting antigen preservation solution, it can ensure that the protein is in a liquid state and is heated at 37 ℃ for 7 days, and the loss of protein activity does not exceed 10 %.
The following uses the company's procalcitonin (PCT) detection kit (latex-enhanced immune turbidimetric method) to test the stability of the antigen. The results show that the accelerated inspection at 37 °C for 7 days, the deviation is ±10% Within 2-8 ℃ storage conditions for 2 months, the deviation is within ± 10%.
37℃ thermal stability
2-8℃ stability
Liding self-produced PCT antigen recommendation:
product name |
English abbreviation |
article number |
PCT antigen ☆ |
PCT Ag |
LD-Ag-PCT |
PCT Antigen Diluent ☆ |
PCT Dilution |
LD-D-PCT-L |
PCT antibodies
product name |
English abbreviation |
article number |
Mouse anti-human PCT monoclonal antibody 01 ☆ |
PCT mAb 01 |
LD-mAb-PCT-01 |
Mouse anti-human PCT monoclonal antibody 02 ☆ |
PCT mAb 02 |
LD-mAb-PCT-02 |
Mouse anti-human PCT monoclonal antibody 03 ☆ |
PCT mAb 03 |
LD-mAb-PCT-03 |
Mouse anti-human PCT polyclonal antibody 04 ☆ |
PCT gpAb 04 |
LD-gpAb-PCT-04 |
Using our company's antibodies can be directly adapted to our company's latex microspheres, and the effect is better!
product name |
English abbreviation |
article number |
Microspheres 400 |
Latex 400 |
LD-cLATEX-400 |
particle size |
380-420 nm |
Carboxyl content |
0.08-0.10mmol/g |
solid content |
10.0-10.5% |
Recommended process
Reagent performance
Features of the top product:
name |
LeadingBio |
Linear range |
0.5-50 ng/mL |
Adaptation parameters |
3:1 etc. (R1/R2) |
sample type |
serum |
Precision |
The intra-assay CV≤5.0%; the inter-assay relative deviation R≤10.0%. |
other |
Can also provide supporting calibrators, quality control, antigen and antibody, etc. |
Linear:
Precision:
|
Inter-assay precision |
within-assay precision |
||
(ng/mL) |
Low |
High |
Low |
High |
N |
10 (batch) |
10 (batch) |
20 times) |
20 times) |
AVE |
0.45 |
30.2 |
0.49 |
31.1 |
CV |
4.7% |
1.4% |
3.0% |
0.9% |
The inter-assay and intra-assay precision of the reagents was good.
Reagent comparison:
Good clinical consistency between reagents and imported reagents
37℃ stability
2-8℃ Stability:
2-8 degrees Celsius open bottle stability
The reagent has good stability, accelerated investigation at 37°C for 7 days, the deviation is within ±10%; 2-8°C open bottle condition is stored for 1 month, and the deviation is within ±10%.
HOOK:
Reagent hook critical concentration ≥ 70ng/mL
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Headquarters: building C6, life science and Technology Innovation Park, Qixia District, Nanjing
E-mail:marketing@leading-med. com
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