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Novel coronavirus neutralizing antibody detection kit (blocking sandwich method)

Novel coronavirus neutralizing antibody detection kit (blocking sandwich method)

Tel:400-0066-825
Keyword:
Novel coronavirus neutralizing antibody detection kit (blocking sandwich method)
Product description

【product name】

Novel coronavirus (SARS-CoV-2) neutralizing antibody detection kit (latex immunochromatography-blocking sandwich method).

【Packaging Specifications】

1 serving/box, 5 servings/box, 20 servings/box.

【expected usage】

This kit is used for the qualitative detection of novel coronavirus (SARS-CoV-2) neutralizing antibodies in human serum, plasma and whole blood (including fingertip blood) samples in vitro.

【Inspection Principle】

This kit utilizes the functional characteristics of the novel coronavirus neutralizing antibody that can block the binding of RBD to the human cell receptor ACE2, and adopts the method of blocking first and then sandwiching to detect the novel coronavirus neutralizing antibody.

 

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The blocking line (B line), test line (T line) and quality control line (C line) of the detection card are coated with ACE2 protein, RBD protein and anti-chicken IgY antibody respectively, and the binding pad contains RBD labeled with red latex microspheres and chicken IgY labeled with red latex microspheres.

When used, RBD labeled with red latex microspheres binds to ACE2 coated on the B line and is captured. When the latex microsphere-labeled RBD protein binds to the 2019-nCoV neutralizing antibody in the sample to form a complex, its binding to ACE2 will be blocked, and the neutralizing antibody on the complex will bind to the RBD coated on the T line. A T-line was formed, and the color shade of the band was positively correlated with the neutralizing antibody titer.

When the sample contains anti-RBD non-neutralizing antibodies, after binding to the RBD labeled with latex microspheres, the RBD will still be captured by the ACE2 coated on the B line, ensuring the specificity of the neutralizing antibody detected by the sandwich method.

Under normal circumstances, the quality control line should be colored to indicate that the test is valid.

【Main components】

1. The kit consists of test card, sample diluent, disposable sterile cotton, blood collection needle, quantitative capillary blood collection tube, etc.

2. The B line, T line and C line of the nitrocellulose membrane of the detection card are respectively coated with ACE2 protein, RBD protein and anti-chicken IgY antibody, and the RBD protein and red latex microspheres labeled with red latex microspheres are sprayed on the binding pad. Labeled chicken IgY antibody.

3. The sample diluent is mainly composed of PBS buffer.

【Storage conditions and validity period】

Store at 1.4~30℃, valid for 12 months.

2. After the test card is opened, please use it within half an hour.

3. See the packaging for the production date and expiration date.

【Sample requirements】

1. This kit is suitable for testing human serum, plasma and whole blood (including fingertip blood). The samples are collected and processed according to conventional methods.

2. Whole blood samples (including fingertip blood) should be used immediately, other samples can be stored at 2-8°C for 7 days, or at -20°C for half a year. Repeated freezing and thawing of samples can degrade test results.

3. Try to avoid severely hemolyzed, lipid-rich or turbid samples.

4. The sample should be fully recovered to 15-30℃ before testing.

【Testing method】

Please read the instruction manual carefully before testing. The samples to be tested, test cards, sample diluents and other testing materials should be equilibrated to 15-30°C 15 minutes in advance and tested under these conditions.

 

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Figure 2: Schematic diagram of the inspection method

1. Open the bag and take out the test card, lay it flat and mark it.

2a. Serum/plasma sample: add 15μL of serum/plasma to the sample addition hole of the test card, and then add 70ul of sample diluent (or drop 4 drops of sample diluent) into the sample addition hole of the test card.

2b. Whole blood sample: use the matching 25μL quantitative capillary tube to draw the whole blood/fingertip blood sample, add it to the sample hole of the test card, and then add 70ul of sample diluent (or use a sample diluent) to the sample hole. 4 drops from a dropper bottle).

3. Interpret the results immediately after 15 minutes.

【Explanation of test results】

 

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Figure 3: Schematic diagram of result interpretation

1. Positive: The result shows two red bands, namely the C line and the T line; it indicates that the sample contains a new coronavirus neutralizing antibody.

2. Negative: Only a red C line appears; it indicates that the sample does not contain novel coronavirus neutralizing antibodies, or the content is lower than the detection limit of the product.

3. Invalid: If the red C line does not appear, it is recommended to use a new test card to re-test.

[Limitations of the test method]

1. This product is limited to qualitative tests for scientific research purposes.

2. Technical or operational errors, as well as other unverified factors that may affect the results, may interfere with the detection and cause erroneous results.

3. In the early stage of viral infection or vaccination, the level of relevant antibodies in the human body is usually very low and cannot be detected immediately. For such test samples, the result may be negative. It is recommended to wait for 2 weeks after the completion of all vaccinations and re-test.

4. Due to individual differences, abnormal test results may occur. If necessary, a comprehensive judgment should be made in combination with the subject's past medical history, physical condition and other diagnostic methods.

【Precautions】

1. This test card is a one-time test product, please do not reuse it; please do not use the product beyond the validity period.

2. Try to use freshly collected samples and avoid using samples with bacterial infection, hemolysis, jaundice or hyperlipidemia.

3. The batch numbers of each reagent component (test card and sample diluent) must be the same.

4. It is recommended to use the test card within half an hour after opening the bag.

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